Bioprinting low viscosity and photopolymerizable human blood derivatives and stem cells

Protein-based hydrogels have great potential to be used as bioinks for biofabrication-driven tissue regeneration strategies due to their innate bioactivity. Nevertheless, they are currently restricted in terms of processability by conventional 3D bioprinting due to their intrinsic low viscosity and liquid character. Inspired by the widely recognized microencapsulation technique, complex coacervation using natural materials can be used to generate microparticles, ultimately composing an efficient multiscale supporting bath.

3 December 2024
10th edition

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Using embedding bioprinting, these protein-based bioinks can be printed within the support bath that efficiently holds the printed filament pattern. Using this strategy, bioprinted constructs fully composed of methacrylated blood derivatives and human mesenchymal stem cells were obtained, without any additive to increase viscosity. These bioactive constructs demonstrated a high-dense cellular network with high cell viability and stability. Due to the universality of this approach, a high number of non-printable human proteins and peptides can be tested, which offers substantial progress for processing clinical grade human components for obtaining human-based tissue like constructs.

A presentation by Monize Caiado Decarli, Postdoctoral researcher at MERLN Institute for Technology-Inspired Regenerative Medicine – Maastricht University.

About Monize Caiado Decarli
She is a biotechnologist with solid background in bioprocess, bioengineering and regenerative medicine with more than 13 years of bench experience. Currently she is post-doctoral researcher at the Institute for Technology-Inspired Regenerative Medicine (MERLN Institute-The Netherlands).
Her areas of expertise are 3D cell models; stem cell differentiation; bioprinting; development of natural hydrogels; osteochondral tissue; bioreactors and production of recombinant proteins.

About MERLN Institute for Technology-Inspired Regenerative Medicine
The MERLN Institute for Technology-Inspired Regenerative Medicine strives to maintain a leading position in the field of biomedical engineering by combining creative research with training a generation of interdisciplinary scientists.

The Institute was founded in 2014 by Prof. Clemens van Blitterswijk. Van Blitterswijk also headed the Complex Tissue Regeneration (CTR) department at that time. Together with the other two departments, namely Instructive Biomaterial Engineering (IBE), led by Prof. Pamela Habibovic and Cell Biology-Inspired Tissue Engineering (cBITE), led by Prof. Jan de Boer, MERLN quickly grew to become a successful institute performing cutting-edge science, establishing new interdisciplinary collaborations, and building up an excellent regional, national and international reputation.
For more information go to the website.

Monize Caiado Decarli is speaker at the 2023 edition of the 3D Medical Conference.